High Resolution Imaging of Radiation Sensitive Biological Samples

Cryo-electron microscopy has seen incredible progress recently. From the first reconstruction of frozen hydrated samples to recent atomicresolution structures, structural biology is undergoing a revolution. However, until recently, the resolution of cryo-EM structures was often significantly lower than that of X-ray crystallography. We have reached a point where, for an increasing number of samples, the resolution achieved using the two techniques are comparable. Atomic scale resolution in cryo-TEM is now possible with the introduction of electron-counting direct detection cameras. We review the progress in the technology of TEM cameras and describe an example of how cutting edge electron counting cameras are being applied in high-resolution cryo-